J.Jpn. Surg. Soc.. 91(1): 36-46, 1990

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Original article

IMMUNOHISTOCHEMICAL STUDY USING A DOUBLE FLUORESCENT STAINING ON THE REGIONAL LYMPH NODES OF GASTRIC CANCERS

For the purpose to investigate the immunological anti-tumor function of lymphocytes in the regional lymph nodes of gastric cancer, a double staining procedure using several monoclonal antibodies against the surface membranes of T and NK cells with fluorescent antibody technique was conducted.
The number of CD11b^＋ cells out of CD8^＋ cells was small with almost all being CD11b-belonging to cytotoxic T cells. Leu8^＋ cells and Leus^－ cells out of CD4^＋ cells were recognized in equal numbers and were reciprocal. The ratio of CD4^＋ Leu8^－/CD4^＋ demonstratedan increase in the group injected with OK-432. The ratio of OKT9^＋ or OKIa1^＋ occupied in CD8^＋ or CD4^＋ cells was only several percent, and few in number. An increase for the ratio of CD4^＋ OKT9^＋/CD4^＋ was observed in the group injected with IL-2. Similar increases for the ratio of CD4^＋ OK1al^＋/CD4^＋ were obtained in the group injected with OK-432 and in the metastatic group, respectively. The Leu7^＋ CD16^＋ cells were not observed. The Leu7^－CD16^＋ cells were observed in a part where metastases were focused.
These results indicated that lymphocytes in regional lymphocyte of gastric cancer might hold the hidden anti-tumor effect, but did not display the full function without preoperative intratumorous injection of BRM such as IL-2 or OK-432.

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