[
Abstract]
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Full Text PDF] （in Japanese / 789KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 91(11): 1736-1739, 1990
Original article
T CELL BLASTOGENESIS IN STORED BLOOD CAN INDUCE GVHD IN OPEN HEART SURGERY
We studied T cell blastogenesis in stored blood during the preservation time within 7 days. Blood samples were obtained from 8 adults and stored at 4℃ immediately after donation with CPD (citrate-phospate-dextrose) for 3, 5 and 7 days. After each intervals, mitogenic responses of T cells from the samples in the presence of an optimal dose of PHA as a mitogen and MLR (mixed lymphocyte reactibn) with lymphocytes prepared by 2000 rad radiation from other 10 donors as stimulating cells were investigated. The stimulation index (S.I.) of PHA as a responsibility of the stored blood was decreasing to 86.7±24.8%, 44.2±12.5% and 31.2±11.1% in proportion of that of fresh blood after 3, 5 and 7 days preservation. The S.I. of MLR were in the same way decreasing to 92.5±21.8%, 45.9±9.8% and 33.3±10.1% after that. These observations suggest that lymphocytes in stored blood within 7 days are able to induce GVHD (graft-versus-host disease) in open heart surgery of which immunological activity is thought to be decreased by extra-corporeal circulation. We concluded that the stored blood within 7 days should be irradiated to 1500 rad or more in order to prevent GVHD.
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