J.Jpn. Surg. Soc.. 87(4): 395-402, 1986

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Original article

CHANGES IN THE ACTIVITIES OF LYSOSOMAL ENZYME (GLYCOSIDASE) IN EXPERIMENTAL ACUTE GASTRIC MUCOSAL LEISION

In order to study the pathogenesis of stress-induced ulcer, we induced experimental acute gastric mucosal lesion (AGML) by loading the rats with heat-burn stress, and we moniterd the activities of four glycosidases having mucolytic action.
The activity of β-N-Acetyl-D-glucosaminidase was 59.50±3.27μg 4MU/h/mg protein before stress, increasing significantly one to three hours after burn, and that of β-N-Acetyl-D-galactosaminidase was 17.50±1.60μg 4MU/h/mg protein before stress, increasing significantly two hours after burn. The activities of two uronic acid exoglycosidases, α-L-Iduronidase and β-D-Glucuronidase were 4.52±0.37 and 8.68±0.89μg phenol/h/mg protein before stress ; both increased significantly one to three hours after burn.
Burn stress caused microcirculatory disturbance in the gastric mucosa, release of histamine and other vasoactive substances, and accelelated release of lysosomal enzymes. Triggerd by these reaction, glycosidases were released to cause the degradation of glycoprotein in the tissue and resulted in the breakdown of the defense mechanisms.

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