J.Jpn. Surg. Soc.. 94(12): 1256-1262, 1993

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Original article

ENDOTOXIN-INACTIVATING ACTIVITY IN NORMAL AND D-GALACTOSAMINE INDUCED LIVER FAILURE RATS

Hepatic failure model rats were prepared by administration of D-Galactosamine (Gal) to clarify the endotoxemia under the hepatic failure. Endotoxin was injected from the femoral vein and the endotoxin level in blood was measured periodically in normal and Gal induced liver failure rats. Further endotoxin was added to plasma, incubated and periodically the endotoxin level in plasma was measured. After the injection of endotoxin, liver biopsy was made and by immunohistochemical staining with Factor C, the localization of endotoxin in the liver was studied. The endotoxin level in blood before the injection was within the normal range in both groups. In the in vivo test, the endotoxin level of the Gal group was significantly higher and its disappearance rate was also significantly lower than the control after the injection of endotoxin. In the in vitro test, the endotoxin level of the Gal group was significantly higher. After one hour, endotoxin was stained on Kupffer cells and hepatocytes in the control group only. In the Gal group, no intrinsic endotoxemia was noted, but it was presumed that endotoxemia is easily caused due to the lowered inactivation of endotoxin in plasma and in the liver.

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