J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 353KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 94(4): 329-333, 1993

Original article

THE QUANTITATIVE ASSAY OF (1-3)-β-D-GLUCAN IN CULTURE MEDIUM OF CANDIDA ALBICANS USING G-TEST

1) Department of Surgery II, Nagoya University School of Medicine, Nagoya, Japan
2) Laboratory of Medical Micology, Nagoya University School of Medicine, Nagoya, Japan
3) Seikagaku Kogyo Co. Ltd., Tokyo, Japan

Hideyuki Kato1), Akimasa Nakao1), Toshio Kambe2), Kenji Tanaka2), Hiroshi Tamura3), Shigenori Tanaka3), Hiroshi Takagi1)

It has been difficult to diagnose the deep-seated fungal infection. Limulus test which orginally has been developed to detect endotoxin in blood is also activated by (1→3)-β-D-glucan, the cell wall component of the fungi. Factor G in limulus lysate is activated by (1→3)-β-D-glucan and not by endotoxin. The quantitative assay of (1→3)-β-D-glucan is possible by the G-test using factor G. (1→3)-β-D-glucan in RPMI culture medium of Candida albicans was periodically measured using G-test and the effect of antifungal drug or neutrophil to the changes of (1→3)-β-D-glucan in the culture medium was studied. Increase in the level of (1→3)-β-D-glucan was in parallel with the growth of Candida albicans. G-test may be applied to the clinical diagnosis of fungal infection.

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