[
Abstract]
[
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[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 93(3): 288-294, 1992
Original article
EFFECT OF PROSTAGLANDIN E1 DERIVATIVE ON LABILIZATION OF LIVER LYSOSOMAL MEMBRANE IN PARTIAL LIVER ISCHEMIA
The change of liver lysosomal enzymes in tissue and serum during a reperfusion period was studied in partial liver ischemic model in rats and effect of Prostaglandin E
1 (PGE
1) derivative on partial liver ischemia was investigated. Partial liver ischemia was induced by clamping the branches of the vessels to the right and caudate lobes of rat liver. The clamp was released after 30 minutes of ischemia. Ischemic and nonischemic lobes of the liver were separately removed and the serum was also collected immediately and two hours after the release of the clamp. Lysosomal enzyme activities from free and bound lysosomal fraction were measured separately and the fragility index (F.I.) was calculated. PGE
1 derivative was administered intraperitoneally 24, 6, 0.5 hours prior to the induction of ischemia at each dose of 0.05 μg/kg. Pretreatment with PGE
1 derivative prevented lysosomal labilization in ischemic lobe, since there was a significant decrease in F.I. of cathepsin D in the PGE
1-pretreated group (preischemia; 28.3±2.4%, immediately after reperfusion; 30.3±2.5%, two hours after reperfusion; 30.3±2.5%) compared to the placebo group (immediately after reperfusion; 40.9±3.4%, two hours after reperfusion; 41.7±3.4%, p＜0.05, p＜0.05, p＜0.01, respectively).
Pretreatment with PGE
1 derivative also significantly suppressed the increase of serum lysosomal enzyme activity. These results showed that PGE
1 derivative improved liver lysosomal labilization in partial liver ischemia.
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