J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 1139KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 93(1): 62-70, 1992

Original article

IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDY ON THE ISLET OF AUTOTRANSPLANTED PANCREAS IN DOGS

1) First Department of Surgery Faculty of Medicine, Kyoto University, Kyoto, Japan
2) College of Medical Technology, Kyoto University, Kyoto, Japan
3) First Department of Pathology, Faculty of Medicine, Kyoto University, Kyoto, Japan

Hiromu Kaji1), Kazutomo Inoue1), Mitsutoshi Yun1), Kotaro Uchida2), Taketoshi Sugioyama3), Takayoshi Tobe1)

This study was conducted to clarify the quantitative and qualitative changes of the islet cells of Langerhans in the autotransplanted pancreas. The left lobe of the canine pancreas was autotransplanted to the left iliac fossa with pancreatic duct left open to the peritoneal cavity. Fibrosis of transplanted pancreas progressed after transplantation. B-cell ratio was decreased significantly (p＜0.01) whereas A-cell ratio and D-cell ratio showed no significant changes. Ultrastructural study revealed the increase of collagen/bundle, degranulation of B-cells and well preservation of A-cell granules at 3 weeks after operation. B-cells with dark cytoplasm were found at 7 weeks after operation which indicate the dysfunction of B-cells. In contrast, clusters of immature B-cells appeared on some sections obtained from the pancreas 14 weeks after autotrasplantation. On the other hand, K-values were decreased significantly 7 weeks after operation (p＜0.05). ΣBS (integrated response value of serum glucose level during IVGTT) was increased significantly (p＜0.01) in addition to the decrease of K-values (p＜0.01) at 11 weeks after operation. It proved that any indices at 14 weeks after operation showed no significant changes from the control group. It might be possible to speculate that relatively well preservation of glucose tolerance at 14 weeks after operation is associated with some clusters of immature B-cells observed ultrastructurally.

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