J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 2068KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 92(2): 113-117, 1991

Original article

A STUDY OF RESISTANCE TO ANTISEPTICS OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN GASTROENTEROLOGICAL SURGERY

First Department of Surgery, Hiroshima University School of Medicine, Hiroshima, Japan
*) Department of General Medicine, Hiroshima University Hospital, Hiroshima, Japan

Yoshio Takesue, Takashi Yokoyama*), Takashi Kodama, Yuji lmamura, Yoshiaki Murakami, Hitoshi Sewake, Yuichiro Matsuura

Highly methicillin-resistant Staphylococcus aureus (H-MRSA, MIC>100μg/ml) was prevalent from 1986 in our institution. The failure of povidone-iodine to reduce the prevalence of MRSA led us to choose chlorhexidine-ethanol solution as an antiseptic,and then the isolation frequency of H-MRSA decreased significantly in 1988. When H-MRSA began to increase again recently, we studied the resistance to antiseptics of MRSA in order to investigate the cause of this re-increase.
Common antiseptics were tested against 45 strains of H-MRSA and 22 strains of methicillin sensitive S. aureus (MSSA, MIC<12.5μg/ml). Dilute preparations (1:100) of povidone-iodine and chlorhexidine･ethanol solution were more effective on H-MRSA than the other antlseptics. Though there was no significant difference between H-MRSA and MSSA in their sensitivity to povidone-iodine, the kllling of H-MRSA strains was more delayed than the killing of MSSA strains in chlorhexidine. Even after a 120-second exposure, 13.3% of H-MRSA strains were resistant to chlorhexidine (more than 1000 colonies were recovered). These highly chlorhexidine-resistant strains have been isolated since 1987 when we chose chlorhexidine-ethanol solution as the antiseptic in our institution. Therefore we suspect that the acquirement of resistance to antiseptics by H-MRSA caused the re-increase of this strain.

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