[
Abstract]
[
Full Text PDF] （in Japanese / 3677KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 91(6): 718-728, 1990
Original article
HUMAN BREAST CANCER CELLS UNDER SERUM-FREE CULTURE. ITS HORMONE-DEPENDEMCY AND APPLICATION TO THE PRIMARY CULTURE
Assessment of hormone-dependency of breast cancer cells in human to estradiol (E
2) and progesterone (Pg)has been made by making serum-free culture of the cells, and primary culture of breast cancer cells is made by this method. The results are as follows: 1. E
2 promotes proliferation of estrogen receptor (ER)-positive cells in low concentrations but rather inhibits it in high concentrations. This inhibitory effect is also observed in ER-negative cells. If the mode of this inhibitory effect is compared with thecellular dynamics of Tamoxifen (TAM) which binds predominantly to ER, the cellular dynamics of E
2 in high concentrations resembles that of TAM, from which some influence being exerted on the binding site of TAM by high concentrations of E
2 is presumed. 2. At binding of E
2 to ER, S phase fraction increases significantly for 24 hours of culture if analyzed from the cellular dynamics, proving that the binding occurs comparatively in a short period. 3. Inhibition of cellular proliferation by PgR depends on the concentration. PgR is not induced with only E
2, and interference of other factors is suggested in the induction. 4. Proliferation of the initially cultured cells is promoted by E
2 although ER is present or not.
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