J.Jpn. Surg. Soc.. 90(7): 1019-1025, 1989

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Original article

BENEFICIAL EFFECTS OF GLUCAGON-INSULIN INFUSION ON HEPATIC PROTEIN SYNTHESIS AND DNA SYNTHESIS IN PARTIAL HEPATIC ISCHEMIA

To study the effects of glucagon-insulin (G-1) infusion on protein synthesis and DNA synthesis in a condition with partial hepatic ischemia, G-I or saline was infused via portal vein for 40 minutes before and after a period of partial hepatic ischemia or following a period of partial hepatic ischemia.
Protein synthesis was measured by ¹⁴C-leucine incorporatin into proteins in incubated liver slices and DNA synthesis by ³H-thymidine incorporation into DNA. Protein synthesis in the postischemic liver was significantly recovered faster and more completely in G-I treated rats. GI infusion enhanced the DNA synthesis of postischemic liver significantly, peaking 48 hours after the period of partial hepatic ischemia. However, the dosage of G-I infused in this investigation couldn't increase the hepatic tissue blood flow measured by hydrogen gas clearance method.
On the histological examination, mitotic index was significantly higher in G-I treated group than in control.
These results suggest that G-I infusion could be beneficial effects on the liver in situation with partial ischemic injury and that G-I infusion could remarkably augment hepatic tissue repair following ischemic damage.

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