J.Jpn. Surg. Soc.. 90(6): 819-825, 1989

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Original article

STUDY OF In Vitro OK432-INDUCED IFN-γ PRODUCTION IN PATIENTS WITH GASTRIC AND ESOPHAGEAL CANCERS : COMPARISON WITH In Vitro SKIN TESTS

In the present study, we measured in vitro production of IFN-γ induced by OK432 in peripheral mononuclear cells (PMC) and investigated the relationship between the skin reaction to Su-Ps, an extract of Streptococcus Pyogenes,and purified protein derivative (PPD) and the producibility of IFN-γ in patients with gastric and esophageal cancers. PMC isolated with the Ficoll-conray centrifugation technique were adjusted to 106 cells/ml in RPMI-1640 medium supplemented with 10％ fetal calf serum and incubated over 7 days at 37℃ in the presense of OK432 at 0.17KE/ml in microcultue plates. IFN-γ secreated in the supernatants was measured consecutively during the observation period with radioimmunoassay. The results are summarized as follows.
1. The production of IFN-γ in PMC from patients with negative skin reaction to Su-Ps and PPD was significantly decreased as compared with the value obtained from patients who were positive in these skin tests (p＜0.001).
2. In vitro production of IFN-γ induced by OK432 was significantly correlated with the degree of skin tests to Su-Ps and PPD as well (r＝0.48, r＝0.41, p＜0.01, respectively).
Thus,it is concluded that the assay of IFN-γ produced in PMC cultures is useful to evaluate the immunological status of patients with cancer.

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