J.Jpn. Surg. Soc.. 89(8): 1204-1210, 1988
Original article
EFFECTS OF VARIOUS ENERGY SUBSTRATES ON THE RECOVERY OF THE
HEPATIC ENERGY LEVEL AFTER COLD STORAGE
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The Second Department of Surgery, Ehime University School of Medicine, Ehime, Japan Yuji Watanabe |
1) Hepatic energy charge (EC) changed from 0.85 (normal value) to 0.42 after the end of 2 hour's cold strage. Hepatic EC recovered partially to about 0.65 due to reperfusion with the perfusate containing glucose (100mg/dl). However, hepatic EC recovered no longer even with the perfusate containing glucose (200mg/d1).
2) Hepatic EC recovered to almost normal value with the perfusate containing 3.3mM alanine and glucose (100mg/dl). In addition, the same recovery was observed with the perfusate containing 0.5mM palmitate and glucose (100mg/dl). At the same time, increase of oxygen consumption was observed due to the addition of alanine on palmitate as compared with glucose in the perfusate.
39 No recovery was observed with the perfusate containing 1mM palmitate and glucose. Also, more increase was observed in the oxygen consumption with the perfusate containing 1mM palmitate than 0.5mM palmitate. It is likely that uncoupling of mitochondria happened by high concentration of palmitate. As a result, glucose ise is not sufficient for the recovery of hepatic EC after cold storage, however, physiological concentration of alanine or palmitate seemed to promote the recovery of the energy level to almost the normal level.
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