J.Jpn. Surg. Soc.. 89(7): 1075-1082, 1988

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Original article

ESTABLISHMENT AND CHARACTERIZATION OF HUMAN LUNG ADENOCARCI-NOMA CELL LINE KUM・LK-2 WHICH PRODUCE SPONTANEOUS LUNG METASTASIS BY NUDE MICE SUBCUTANEOUS IMPLANTATION

Characterization were newly established at the human lung adenocarcinoma cell line KUM • LK-2 which produced spontaneous lung metastasis by subcutaneous implantation in nude mouse. KUM • LK-2 cell line was established from the 2nd generation of nude mouse serially transplanted human lung adenocarcinoma. KUM • LK-2 produced spontaneous lung metastasis when cells were implanted into subcutaneous tissue of nude mice. Metastasis was not found in the cases of intramuscluar implantation and intravenous injection of these cells. KUM • LK-2 cell produced Carcinoembryonic Antigen and Carbohydrated Antigen 19-9 in vitro, but these tumor markers were not detected in the sera of KUM • LK-2 transplanted nude mice. From this study the model of human cancer with spontaneous lung metastasis was established. It was suggested that metastasis of human cancer in the nude mouse could not be determined by blood supply and new vascular genesis only. And it was concluded that usefulness of nude mice as an amplifier of tumor marker is varied with tumor lines.

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