J.Jpn. Surg. Soc.. 87(3): 341-349, 1986
Original article
COMPETITIVE BINDING ASSAY WITH DEXTRAN COATED CHARCOAL FOR MEASUREMENT OF SEX HORMONE BINDING GLOBULIN IN BREAST CANCER
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Second Department of Surgery, Tokushima University School of Medicine, Tokushima, Japan Yoshihisa Suminaga, Hiroshi Sonoo*), Tadaoki Morimoto |
This method is based on the estimation of the amount of estradiol (E2) bound with SHBG which possessed binding sites with it. After removing the endogenous steroids from the serum-samples by adsorption with dextran coated charcoal, the value of SHBG-BC increased by 18％ compared with that of untreated serum.
In 48 patients of various ages with breast cancer, the value of SHBG-BC (34.6±11.1 ; mean ±S.D.), expressed as pico mole per 1ml serum, die not differ significantly from that of 12 healthy control women (29.6±6.6). However, in postmenopausal women, the value of SHBG-BC in 24 patients with breast cancer (33.4±12.2) differed from that of 6 controls (26.1±4.9 ; p＜0.05).
In addition, estrogen receptors (ER) were measured by using gel-filtration me thod in cytosols from the tissue of breast cancer. The value of SHBG-BC in 15 ER-postive cases (40.4±10.1) were significantly higher than that in 13 ER-negative cases (29.3±8.7 ; p＜0.01), but the value of SHBG-BC and ER did not run parallel completely.
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