J.Jpn. Surg. Soc.. 86(4): 400-403, 1985

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Original article

ALANINE METABOLISM IN SKELETAL MUSCLE AND PLASMA IN ENDOTOXEMIA

Utilizing a 24 hour fasting rabbit (N＝30), we measured free amino acids in the femoral artery and vein and the quadriceps femoris muscle. The endotoxin E. coli 026, 3.0mg/kg (LD₁₀₀) was injected and free amino acid plasma levels were monitored for 6 hours.
1) Changes in free amino acid plasma levels were variable and marked after endotoxin injection.
2) By 360 min. after endotoxin injection : (a) the rate of increase in free amino acid levels in the femoral artery was 366μmole/l of alanine, 162μmole/l of glycine and 85μmole/of proline ; (b) the rate of increase in free amino acid of the quadriceps femoris muscle was 1376 nmole/g of alanine, 156 nmole/g of glycine and 109 nmole/g of serine ; and (c) the femoral arteriovenous difference was －225 nmole/l of alanine, －118 nmole/l of glycine and －77nmole/l of proline.
3) Within 10 min. after endotoxin injection, alanine concentration was higher in the femoral vein. This change in concentration became significant by 60 min.
The results show the following : Skeletal muscle appears to be an important source of amino acids for amino acid metabolism during endotoxemia, especially plasma alanine which is closely connected with alanine levels in skeletal muscle.

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