[
Abstract]
[
Full Text PDF] （in Japanese / 4679KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 86(1): 44-50, 1985
Original article
A COMPARATIVE STUDY OF EXPERIMENTAL MODELS FOR CARDIAC PRESERVATION THROUGH ORTHOTOPIC TRANSPLANTATION
Various kinds of models for canine heart preservation were evaluated through orthotopic transplantation. Following warm ischemic (WIT) and preservation times (PT), donor hearts were orthotopically transplanted to recipients and observed for 2 hours. The study was divided into four groups. A heart-lung preparation (Group A) was preserved by coronary perfusion with diluted blood for an hour following 15-minute WIT in A-1 (n＝33), and 2 hours following 30-minute WIT in A-2 (n＝19). Following 30-minute WIT, the heart (Group B) was preserved for an hour by coronary perfusion with diluted blood in B-1 (n＝20), a modified Krebs solution in B-2 (n＝14), and a EL-solution in B-2 (n＝14). Following 30- minute WIT, the heart (Group C) was preserved for 2 hours by a Langendorff’s model with coronary perfusion using perfluorochemical in C-1 (n＝5) and Hydroxyethyl starch in C-2 (n＝1). In Group D, electromechanical arrest of the heart and coronary vascular washout were performed in D-1 (n＝8) with K
＋ cardioplegia, D-2 (n＝8) with K
＋-verapamil cardioplegia, and D-3 (n＝5) with Collins M-verapamil cardioplegia. The heart was then removed and suspended in the same solution. The graft was preserved at 4℃ for 6 hours in D-1 and D-2, and 24 hours in D-3.
Fourty-eight ％ in A-1 and 26％ in A-2 were successfully transplanted. In B and C groups, 80, 64, and 79％ in B-1, B-2 and B-3, respectively, and 80 and 0％ in C-1 and C-2, respectively were successfully transplanted. Meanwhile, successful ratio in D group was 63, 100, and 100％ in D-1, D-2 and D-3, respectively. The present study suggests that the combination of coronary vascular washout with K
＋- verapamil cardioplegia and single storage at 4℃ with the same solution is effective for myocardial protection of the canine heart through transplantation.
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