J.Jpn. Surg. Soc.. 85(6): 534-540, 1984
Original article
EXPERIMENTAL STUDY ON THE OPTIMAL POTASSIUM AND MAGNESIUM CONCENTRATION IN THE CARDIOPLEGIC SOLUTION
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Second Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan Junichiro Takahashi, Masamori Matsuura, Fujio Watanabe, Toshikazu Tachiki, Hiroshi Edasawa, Toshiaki Kawakami, Tatsuzo Tanabe |
The perfusion of infusate of 15 mM per liter of K-aspartate stopped the heart beat completely, and as the same way it was necessary 25 mM per liter of MgCl2 to get cardiac arrest. By their combination, however, heart was arrested with infusate of 10mM per liter of K-aspartate and 15mM per liter of MgCl2, which were lower concentration than K-aspartate or MgCl2 alone.
In order to determine the optimal concentration of potassium and magnesium in cardioplegic solution, the isolated rat hearts were presserved in relatively disadvantageous condition; such as 37℃ of infusates, non-oxygenation, continuous perfusion and perfusion pressure 50 cmH2O during 120 minutes.
Sixty isolated rat hearts were divided into eleven groups. Each group received a different proper combination of KCl (5.9, 15, 25, 40, 60 mM/L) and MgCl2 (1.2, 13, 25, 33, 50 mM/L) in cardioplegic solution.
Using the isolated working rat heart apparatus, hemodynamic indices after 120 minutes preservation were compared with control values of the same hearts, and their percent recoveries were compared with one another.
In conclusion it appears that in the isolated working heart model combination of KCl 40 mM/Land MgCl2 13mM/L in Basic-Modified-Krebs-Solution might offer the best myocardial protection of all combinations tested.
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