J.Jpn. Surg. Soc.. 82(7): 759-772, 1981

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Original article

STUDY ON THE EFFECT OF ANTI-ALPHA-FETOPROTEIN ANTIBODY ON AFP PRODUCING RAT HEPATOMA

It has been reported that anti-alpha-fetoprotein serum (AAA) inhibits proliferation of AH66 rat hepatoma cells in vitro. In this study, in vivo effect of AAA on alpha-fetoprotein (AFP) producing hepatoma was investigated by the method of injection of AAA into tumor vessels of 3'-methyl-4-dimethylaminoazobenzene (DAB) induced rat hepatoma and AH66 implanted rat hepatic tumor. Sham operation and injection of normal horse γ-globulin were done as control. Following results were obtained.
No influences on survival days, tissue AFP levels and histopathological appearences of DAB rat hepatoma were found. No allergic reactions were observed in lung, spleen and kidney of those rats in which AAA was administered. By immunofluorescence technic, AAA injection groups showed slightly decreased AFP immunofluorescence compared with control groups. In group of 20 weeks administration of DAB, serum AFP levels were depressed during a week after injection, and during 5 weeks depression was found in 15 weeks DAB administration group. Within the limit of 110 mm³ tumor volume, AH66 implanted hepatic tumors were depressed its enlargement by administration of high dose of AAA.
In conclusion, anti-rat-alpha-fetoprotein has effects of depression of serum AFP level and suppression of growth of small size of AH66 implanted rat hepatic tumor.

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