J.Jpn. Surg. Soc.. 81(11): 1423-1436, 1980

---

Original article

THE LEUKOCYTE MIGRATION INHIBITION TEST TO TUMOR ASSOCIATED ANTIGENS IN BREAST CANCER PATIENTS

The direct leukocyte migration inhibition test was used to measure the cellular immune response in breast cancer patients to various type of tumor associated antigens (TAA), such as autologous and homolougous breast cancer tissue, two different human breast cancer cell lines (MCF-7, CAMA), mouse mammary tumor virus (MuMTV) and Mason-Fizer virus (MPV). Normal breast tissue, cancerous and normal colon tissue and mouse leukemia virus (MuLV) were also evaluated in comparison to TAA. Patients with benign breast tumor, head and neck cancer, and normal women were tested.
Breast cancer patients failed to show significant positive response to autologous TAA. While, there was a significant high frequency in response to homologous TAA and MCF-7. Low response rate to CAMA in breast cancer patients suggested a quantitative and qualitative difference in antigenicity among cell lines. Leukocyte from breast cancer patients showed a high frequency in response to MuMTV, but did not to MPV or MuLV. Simultaneous tests to two cel lines and three different viruses showed that a large number of breast cancer patients highly responded to two antigens as MCF-7 and MuMTV. Responses to the antigens pair (MCF-7, MuMTV) suggested the similarity in antigenicity between them.
Our data may indicate the existence of an immune response associated with TAA, stimulating to the leukocytes in same breast cancer patients, and offer an immunological assay system for diagnosis of breast cancer in the future.

---

---

To read the PDF file you will need Adobe Reader installed on your computer.