J.Jpn. Surg. Soc.. 81(7): 581-593, 1980
Original article
INFLUENCE OF ENDOTOXIN ON ENERGY METABOLISM
－HEPATIC MITOCHONDRIAL FUNCTION AND ADENINE NUCLEOTIDES－
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Department of Surgery II, Kanazawa University, School of Medicine, Kanazawa, Japan Hiroshi Ueda |
1. In vitro effects of LPS
An addition of 200 μg LPS per mg mitochondrial protein to the isolated rat liver mitochondria increased the state 4 respiratory rate, which indicated that LPS had uncoupling activity in the form of ATP.
2. In vivo effects of LPS
Initially, mitochondrial RCR increased and hepatic ATP level was remain constant. In the late stage, RCR and ADP/O ratio were decreased, in which condition ATP level, total adenine nucleotide and energy charge were estimated to be decreased.
3. The effects of LPS given in perfused rat liver
In order to avoid the effects of other factors, liver perfusion of Mortimore type was used. In perfused rat liver with infusion LPS at normal flow rate, there were no significant differences in adenine nucleotide levels and energy charge comparing to the control without LPS infusion. Reduction of the flow rate, however, resulted in marked decrease in ATP level and total adenine nucleotide, in which energy charge was decreased.
It is concluded that the disturbed hepatic energy metabolism in endotoxin shock is mainly caused by tissue hypoxia.
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