J.Jpn. Surg. Soc.. 81(2): 119-132, 1980

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Original article

EXPERIMENTAL STUDIES ON REGULATION OF SERUM FREE AMINO ACIDS IN LIVER FAILURE

Adult mongrel dogs were divided into 3 groups. Four dogs were in group I (G-I), where no surgery was performed. Group II (G-II) and group III (G-III) had 4 dogs each,who were subjected for the study 2 and 4 weeks after ligation of the cystic and common bile ducts, respectively. Solution of various amino acids (AAs) composition was continuously drip infused for 3 hours via cathter placed in the mesenteric vein. Blood and urine samples were periodicaly obtained during the time from the portal vein (P), hepatic vein (H), femoral artery (A), and urether (U) for analysis of free AAs profile.An uptake ratio for each amino acid (AA) by the liver was calculated by
(1-5C_H/C_A+ 4Cp),
where C represents concentration of a given AA. A retention rate(%) was also calculated for each AA by
(loaded AA-C_U/loaded AA×100),
The following results were obtained.
1) CA before loading is the highest in G-III followed by G-I and G-II.
2) The uptake ratio in G-III is higher than that in G-I and G-II for the initial 1 hour, then ths was reversed.
3) The average uptake rate for glycine, alanine and serine were the highest in G-II, and that of arginine in G-III.
4) The retension rate did not show significant difference between G-II and G-III.
These results indicate that 1) ability to regulate serum AAs levels in G-III is lower than that in G-I and G-II, because of poor hepatic uptake rate, and 2) C_A, hepatic uptake of AAs and the retension rate of AAs can serve as indicators to select appropriate composition of AAs in treating metabolic derangement in liver failure.

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