J.Jpn. Surg. Soc.. 96(12): 799-810, 1995
Original article
HISTOLOGICAL STUDY FOR ENDOTHELIAL SEEDED VASCULAR PROSTHESES-EXPERIMENTAL STUDY ON CAINE
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The Second Department of Surgery, Faculty of Medicine, University of Tokyo, Japan Jun Egami |
In this report, we established endothelial lining onto ultrafiber graft by the filtration method and implanted it to caine abdominal aorta. The grafts were harvested in 1 to 4 months and examined endothelialization and subendothelial structure by light microscopy and electron microscopy.
The cell counts of pre and post flush through the graft were caluculated. pre : 9.8±106/ml post : 1.2± 0.60×106/ml trap ratio : 86.4±4.6%. The thicness of inner capsule of seeding part were significantly low than that of control part. But there were no significant difference of inner capsule of anastomosis between seeding part and control part.
In the seeded part endothelial lining and well incorporation were observed by light microscopy. Electronmicroscopic study revealed that seeded endothelium had desmosome and secreted a basement membrane. In the control part there was only small endothelial inoculation and thrombus formation which caused stenosis of graft. The filtration method revealed the efficacy to seed on to vascular prosteses for long time period. The seeded graft had antithrobogenity and well incorporation.
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