J.Jpn. Surg. Soc.. 96(5): 301-308, 1995
Original article
HEPATOCELLULAR TRANSPLANTATION IN RATS WITH CONGENITAL ASCORBIC ACID DEFICIENCY
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Second Department of Surgery, Asahikawa Medical College, Japan Kazuhiko Onodera, Shinich Kasai, Michio Mito |
Hepatocytes were isolated from ODS-+/+rats, which are congenic to ODS-od/od rats and have hepatic L-gulonolactone oxidase. A total of 1×107, 1×107, and 2.5×106 hepatocytes were respectively transplanted into the peritoneal cavity, spleen, or portal vein of ODS-od/od rats, which are unable to synthesize ascorbic acid (AsA) due to Iack of hepatic L-gulonolactone oxidase. After 4 days of oral pretreatment with 0.05% 2-acetylaminofluorene, recipients underwent 70% partial hepatectomy just before transplantation. AsA administration was discontinued at 6 weeks after transplantation. The symptom-free survival rate and the serum AsA level of recipient rats were determined at 6 weeks after discontinuing AsA administration.
The symptom-free survival rate of untrasplanted rats and recipient rats with intraperitoneal, intrasplenic and intraportal hepatocyte transplantation were 0%, 0%, 60%, and 100%, respectively. The serum AsA levels were 0.20±0.20μg/ml, 0.14±0.05μg/ml, 1.06±0.26μg/ml, and 1.58±0.61μg/ml, respectively. Intrasplenic or intraportal transplantation was able to cure ODS-od/od rats. A subsequent splenectomy study showed that hepatocytyes reaching the liver via the splenic vein following intrasplenic hepatocyte transplantation played a major role in this experimental success.
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