J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 2962KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 94(12): 1269-1276, 1993

Original article

THE ROLE OF CA2+-ATPASE AND OXYGEN RADICAL IN REPERFUSION INJURY OF RAT LIVER

1) First Department of Surgery, Nippon Medical School, Tokyo, Japan
2) Second Department of Pathology, Nippon Medical School, Tokyo, Japan

Hitoshi Watanabe1)2), Masahiko Onda1), Atsushi Genga1)2), Goro Asano2)

The effects of ischemia and reperfusion with and without oxygen radical scavengers and xanthine oxidase inhibitors on Ca2+-ATPase activity were examined in the rat liver of 5 min ischemia followed by 5 and 10 min reperfusion. Ischemia was produced by the ligation of right hepatic artery and right portal vein. Superoxide dismutase, catalase and allopurinol were administered by subcutaneous injection of 60,000U/kg, 90,000U/kg and 200mg/kg, respectively before ligation. Reaction products of Ca2+-ATPase were morphometrically analyzed by RUZEX IIIU. Histochemically, Ca2+-ATPase activities were demonstrated on plasma membrane of liver cells, bile canaliculi and Kupffer cells involving mitochondria in liver cells of control rats. Ca2+-ATPase activities were depressed in the central lobes of liver after 5 min ischemia followed by 5 and 10 min reperfusion. However, the activities of Ca2+-ATPase were not depressed by addition of oxygen radical scavengers and xanthine oxidase inhibitor before ischemia. These results suggest that oxygen free radicals may influence Ca2+-ATPase activity and contribute to liver cell damage due to ischemia-reperfusion.

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