[
Abstract]
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[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 94(9): 1033-1042, 1993
Original article
THE EFFECT OF OXYGEN SUPPLY IN CONTINUOUS COLD PERFUSION OF THE RAT LIVER USING PERFLUOROCHEMICAL EMULSION
―PARENCHYMAL AND NONPARENCHYMAL CELL INJURIES EVALUATED WITH TRYPAN BLUE PERFUSION/FIXATION TECHNIQUES
We investigated the effect of hypothermic aerobic perfusion of the rat liver using perfluoro-N-methyl-decahydroisoquinoline (FMIQ) on the cell viabilities of hepatic parenchyma and nonparenchyma in comparison with University of Wisconsin (UW) solution. Rat liver were perfused at 4°C up to 24hr at a flow rate of 0.2ml/g liver tissue/min with either UW or a perfusate (m-FMIQ) containing 20% FMIQ with or without oxygen saturation. After storage, livers were perfused with Krebs-Henseleit buffer containing trypan blue (200 μM). Nuclear dye uptake was mesured as an indicator of cell death. Dye uptake of parenchymal cells remained less than 2% for up to 24hr, regardless of perfusate or O
2-saturation. In UW perfused livers, nonparenchymal cells showed time related increases of dye positive nuclei,which were not affected by O
2-saturation (39.9±4.7% and 36.5±4.2% at 24hr in O
2-saturated and nonsaturated groups, respectively). In contrast, we found excellent protective effects of oxygen supply with m-EMIQ on the nonparenchymal cell viability, the dye uptake values being 16.9±5.7% and 39.4±9.1% at 24hr in O
2-saturated and nonsaturated groups, respectively (p＜0.001). In conclusion, oxygen supply during hypothermic perfusion may be useful in preventing nonparenchymal cell injury of the liver, thereby leading to dramatic improvement of the hepatic microcirculation.
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