J.Jpn. Surg. Soc.. 94(8): 863-868, 1993
Original article
EFFECT OF Na-L-GLUTAMATE ON MITOCHONDRIAL FUNCTION OF ATP SYNTHESIS AFTER PROLONGED PRESERVATION IN RAT HEART
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1) Second Department of Surgery, Miyazaki Medical College, Miyazaki, Japan Masachika Kuwabara1), Toshio Onitsuka1), Koichiro Shibata1), Yasunori Koga1), Minoru Hamada2) |
We divided isolated rat hearts into the following three groups : group A, hearts without immersion as control ; group B, hearts immersed in Euro-Collins solution (E-C) without Glu for 24 h at 4°C; group C, hearts immersed in E-C with 10mM Glu for 24 h at 4°C. Number of each group used was 6.
Mitochondrial functions of ATP production were determined by measuring respiratory control index (RCI). P/O ratio and state III respiration rate. The ability of ATP production (oxidative phosphorylation rate, OPR) was calculated.
RCI and P/O ratio of groups B and C were significantly lower than those of group A. RCI and P/O ratio of group C were significantly higher than that of group B. S3 rates of groups B and C were significantly lower than those of group A. S4 rate of group B was significantly higher than those of groups A and C.
OPRs of groups B and C were significantly lower than that of group A. OPR of group C was significantly higher than that of group B.
We concluded that Na-L-glutamate effectively protected mitochondrial functions to synthesize ATP during prolonged hypothermic preservation.
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