J.Jpn. Surg. Soc.. 94(6): 593-603, 1993

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Original article

EXPERIMENTAL STUDY OF THE TIME COURSE OF DNA STRAND BREAKS AND DNA SYNTHESIS DURING LIVER REGENERATION AFTER PARTIAL HEPATECTOMY IN RATS

The time course of DNA strand breaks (nicks) and DNA synthesis during liver regeneration were studied in rats with 70% partial hepatectomy. Cellular nicks were detected by in situ nick translantion (ISNT) using biotinlabelled mixture and DNA synthesis was assessed by immunohistochemical staining using BrdU and/or PCNA monoclonal antibodies.
Nicks appeared on periportal and subcapsular hepatocytes in normal liver tissue, and increased from 24 hours to 48 hours (peak time) after hepatectomy in mainly periportal area. Hepatocytes with BrdU intake increased from 24 hours after hepatectomy with peak value in mainly periportal area. Hepatocytes stained with PCNA antibody increased from 24 hours after hepatectomy in mainly periportal area and there were most positive staining cells at 48 hours after hepatectomy.
In liver regeneration after hepatectomy, it apparently suggested that nicks in hepatocytes appeared in G₀-G₁ phase that was slightly before S phase and after M phase, but almost cells at G₀-G₁ were not stained, and hepatocytes with PCNA protein appeared following the BrdU intake in the same cells.

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