J.Jpn. Surg. Soc.. 94(4): 400-411, 1993
Original article
CULTURE OF HUMAN ADULT ENDOTHELIAL CELLS DERIVED FROM CADAVERS OF AUTOPSY
|
First Department of Surgery, School of Medicine, Tokyo University, Tokyo, Japan Minoru Okumura |
Iliac areteries of cadavers were resected at autopsy and their endothelial cells were yielded sterilely by digestion of 0.1% collagenase solution. I tried culture of endothelial cells of 72 cadavers at autopsy and 14 cases of them were successful.
They were observed with phase-contrast microscopy, transmission electron microscopy and immunofluorescence microscopy, which showed polygonal monolayered cells, Weibel-Palade bodies and Factor VIII antigen in these cells. I also examined the doubling time (2-4 weeks) of these cells. 3H-Thymidine laveling index showed the differentiation of effects between fibroblast growth factor and endothelial cell growth factor on human adult endothelial cells, while no differentiation between them on human unbilical vein endothelial cells. Also, I observed apolipoprotein E in the cells, but not in umbilcal vein endothelial cells. In established the procedure of their culture method.
To read the PDF file you will need Adobe Reader installed on your computer.