J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 2321KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 94(3): 234-241, 1993

Original article

PRODUCTION OF LEUKOTRIENES IN RAT KUPFFER CELLS AND HEPATOCYTES BY VARIOUS INDUCERS

1) Department of Emergency and Critical Care Medicine, Nippon Medical School, Tokyo, Japan
2) The Second Department of Biochemistry, Nippon Medical School, Tokyo, Japan

Yasuhiro Otomo1), Yoshikazu Kanda2), Yoshio Yoshino2), Toshifumi Otsuka1)

In order to verify whether rat hepatocytes are capable of producing leukotrienes, Percoll density gradient centrifugation to fractionize rat hepatocytes and a radioimmunoassay of leukotrienes in the reacting solution have been performed. Also, investigated was effect of retinol (Vitamin A) on inducing the production of leukotrienes in rat Kupffer cells and hepatocytes.
Incubation with endotoxin (10μg/ml) for 30～60 minutes induced the production of leukotrienes in rat Kupffer cells but did not induce any such production in rat hepatocytes. On the other hand, arachidonic acid (2～20μM) or linoleic acid (10～50μm) did induce hepatocytes to produce leukotrienes in a dose-dependent manner. Fifteen minutes of pre-incubation with 10-8M of retinol inhibited an endotoxic induction of leukotrienes production in rat Kupffer cells, whereas it did not inhibit an arachidonic acid induction in rat hepatocytes. Based on these results, we have concluded that a hepatic tissue injury during sepsis is probably mediated by leukotrienes produced by the Kupffer cells and its inhibition is the mechanism of tissue protective effect of retinol. However, in further severe state even the hepatocytes may produce leukotrienes and as a consequence, a widespread destruction of hepatic tissue will occur.

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