J.Jpn. Surg. Soc.. 93(10): 1324-1329, 1992
Original article
THE EFFECT OF HYPERTHERMIA AGAINST PANCREATIC CANCER CELL
―VARIOUS EXAMINATIONS INCLUDING FLOW CYTOMETRIC BROMODEOXYURIDINE/DNA ANALYSIS―
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The First Department of Surgery, Osaka City University Medical School, Osaka, Japan Bunzo Nakata, Yong-Suk chung, Tetsuji Sawada, Hideaki Yokomatsu, Hajime Tanaka, Katsusuke Satake, Michio Sowa |
The hyperthermia above 43°C was thought to have strong effect for the studies on surviving cell number and morphological change of Capan-2 and RWP-1 cells after heating treatment. The cell inoculated to the nude mice subcutaneously after heating treatment at 43°C was recognized the tumor forming at 3 weeks later, however, the growth speed was slower than that of control to which untreated Capan-2 cells were inoculated. As the case of 44°C, the tumor forming was not recognized at all. The cytokinetics was measured by flow cytometric bromodeoxyuridine (BrdUrd)/DNA analysis. Up to 43°C in accordance with increase in the heating temperature, the accumulation in G2M phase increased, and the amount of BrdUrd incorporated 5 phase decrased. At the temperature above 44°C, BrdUrd was hardly incorporated into S phase cells and the cell cycle was almost stopped. These results suggested the usefulness of hyperthermia and strong effect of heating treatment above 44°C against pancreatic cancer cell.
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