J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 585KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 91(7): 901-906, 1990

Original article

ANALYSIS OF CELLULAR DNA-RNA CONTENT USING FLOW CYTOMETRY BETWEEN PRIMARY AND METASTATIC FOCI IN LUNG CARCINOMA

1) Department of Thoracic Vascular Surgery, Oita Prefectural Hospital, Oita, Japan
2) First Department of Surgery, Nagasaki University, School of Medicine, Nagasaki, Japan
3) Department of Surgery, Ureshino National Hospital, Ureshino, Japan

Norio Yamaoka1), Yutaka Tagawa2), Hiroyoshi Ayabe2), Katunobu Kawahara2), Harumi Kida3), Takayoshi Uchiyama1), Masao Tomita2)

By flow cytometry, cellular DNA-RNA content was analyzed with Acridine Orange staining for primary tumor and the metastatic foci in 12 cases of primary lung carcinoma. Five（42％）of the primary and metastatic foci showed heterogeous cellular DNA content, with high cellular DNA content of metastatic foci in all cases.
Cellular RNA content was significantly higher in metastatic foci than in primary foci, indicating possible higher proliferative activity of the former foci. Three different metastatic foci in one patient showed essentially the same cellular DNA content.
From the above it is evident that primary and metastatic foci of lung carcinoma show considerable heterogeneity not only in cellular DNA content but RNA content as well, thus indicating proliferative activity of the metastatic focus. It is consequently advisable to use a combination of various supplementary medications differing in cytocidal effect. Cases with higher cellular DNA content may likely have higher metastatic potential.

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