J.Jpn. Surg. Soc.. 91(3): 303-312, 1990
Original article
THE PERSISTENCE AND PROLIFERATION OF TUMOR-XENOGRAFTS IMPLANTED UNDER THE RENAL CAPSULE OF IMMUNOCOMPETENT MICE, CYCLOSPORIN A-TREATED MICE AND NUDE MICE
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Department of Surgery, Research Institute for Microbial Diseases,Osaka University, Osaka, Japan Takatsugu Kusuyama, Masahide Fujita, Yasushi Okuyama, Tetsuo Taguchi |
Twelve tumor-specimens were implanted simultaneously under the renal space of immunocompetent CDF1 mice, cyclosporin A (CsA) 60mg/kg treated mice, and BALB/c-nu/ nu (nude) mice. The persistence and growth of implanted tumor-xenografts of each mouse, was evaluated, on day 6 and 9 after inoculation. The tumor-xenografts implanted under the renal space of immunocompetent mice, grew larger on days 6 in 9 cases, but histological evaluation showed tumor tissues were in various degree replaced by host reactive tissues. Host reaction in CsA-treated mice or nude mice was suppressed almost completely, but the persistence and proliferation of tumor-xenografts of both mice was varied, depending on the nature of original tumors.
The judgment for cancer chemotherapy on our modified SRC assay was almost similar between CsA-treated mice and nude mice, but there were some cases in which macroscopical judgment didn't correspond with histological effect.
The DNA synthesis of tumor-xenografts of 7 patients, was examined by using sequential changes of BrdU labeling index (LI) in the renal space of CsA-treated mice. It was showed LI rather indicated the nature of original tumors itself.
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