J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 3945KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 90(10): 1732-1741, 1989

Original article

RADIOIMMUNODETECTION OF COLORECTAL CANCER, USING ANTI-CEA MONOCLONAL ANTIBODIES

*) The Second Department of Surgery, Nagoya University School of Medicine, Nagoya, Japan
**) Department of Radiology, Nagoya University School of Medicine, Nagoya, Japan
***) The Third Department of Surgery, Aichi Cancer Center, Nagoya, Japan

Hiroki Murayama*), Tadashi Watanabe*), Junichi Sakamoto***), Masanori Tadokoro**), Hiroshi Takagi*), Sadayuki Sakuma**)

Aiming at radioimmunodetection of colorectal cancer, anti-CEA monoclonal antibodies (CEA102) were produced by immunization with purified CEA.
CEA102 showed high specificity with colorectal cancer by mixed hemadsorption assay and immunoperoxidase technique.
The antigen detected by CEA102 was confirmed to be carcinoembryonic antigen (CEA) and its molecular weight was estimated to be ca. 180,000 by biochemical analysis.
The in vivo study using nude mice grafted a human colorectal cancer or a human malignant melanoma showed greater accumulatin of 125I-labeled CEA102 in CEA-positive colorectal cancer than in nude mouse tissues and CEA-negative malignant melanoma. Moreover we successfully obtained scans with good localization of the grafted colorectal cancer on FCR (Fuji Computed Radiography).
Using 131I-labeled CEA102 liver metastasis in the patient with colorectal cancer was successfully detected by external scanning with γ-camera.
These results suggest that radiolabeled CEA102 is useful for the detection of colorectal cancer.

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