J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 1322KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 90(9): 1576-1578, 1989

Report on the annual meeting

IN SITU DETECTION OF CELLULAR DNA STRAND BREAKS INDUCED BY ANTITUMOR DRUG AND HEAT USING NICK TRANSLATION

1) The Second Department of Surgery, Kyushu University, Fukuoka, Japan
2) Cancer Center of Kyushu University Hospital, Faculty of Medicine, Kyushu University, Fukuoka, Japan

Yoshihiko Maehara1), Hideaki Anai2), Tetsuya Kusumoto2), Yoshihisa Sakaguchi2), Yasunori Emi2), Shunji Kohnoe2), Keizo Sugimachi1)2)

Cellular DNA strand break induced by an alkylating agent : Carboquone (CQ), and heat (43℃) was detected in HeLa cells in vitro and mouse sarcoma-180 cells in vivo. The break sites in the DNA were translated artificially in the presence of Escherichia coli DNA polymerase I and［3H］-labeled dTTP and sites in the DNA were visualized by autoradiographic observation of grains in the nuclei. These breaks increased in a dose and time dependent manner, compared to findings in the control cells.
Our findings show that the surviving response of cells decreases while the level of DNA strand breaks increases following exposure to CQ or heat. The nick translation method is a rapid in situ assay for determining drug and heat induced DNA damage of tumor cells, under in vitro and in vivo conditions and in a Semi-quantitative manner.

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