J.Jpn. Surg. Soc.. 90(5): 745-752, 1989

---

Original article

INHIBITORY EFFECT OF ANTI-PLATELET PROSTAGLANDIN ON LIVER METASTASIS OF HAMSTER PANCREATIC CANCER

Coagulation system and platelets play an important role in the stage of lodgement of tumor cells.
We examined abilities of human and hamster pancreatic cancer cell lines to aggraegate platelets in vitro, and investigated the effect of prostaglandin E₁, I₂, on artificial liver metastases of pancreatic cancer in Syrian golden hamster. P1 atelet aggregating activities were found in five out of six human pancreatic cancer cell line and thromboplastin likes activity in five cell lines. Diisopropanolnitrosamine induced hamster pancreatic cancer cells (HPK-1) were able to aggregate platelets both in vitro and in vivo and these activities were inhibited by prostaglandin I₂. Hamster was inoculated intraportally with 1×10⁶ HPK-1 cells. After two weeks autopsy of these hamsters revealed multiple metastatic nodules on liver surface.
In this model we administered prostaglandin E₁, I₂ into the portal vein five minutes before cell inoculation. Number of liver surface nodules were significantly decreased to 33.1+7.0,11.0+9.6 in hamster given 100g PGE₁ PGI₂ before cell inoculation, compared with control group of hamsters (62.0+ 6.6PH9. 3,66.1+13.9PH7.4). But administration of prostaglandin after cell injection was not effective. In all cases none of exttahepatic metastases were noted.
Inhibitory action of PGE₂ PGI₂ on liver metastasis is suspected to be related to inhibition of platelet aggregation.

---

---

To read the PDF file you will need Adobe Reader installed on your computer.