J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 3083KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 87(3): 266-272, 1986

Original article

THE EFFECTS OF SERUM FROM TUMOR BEARING MICE ON MITOGEN-INDUCED BLASTOGENESIS OF NORMAL SPLEEN CELLS

The Second Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

Kuniyuki Tsuchiya, Tsuguo Tanaka, Seishiro Inaba, Shimpachiro Nomi, Kenichiro Hamagashira, Haruki Hamada, Takahiro Oka

In order to detect the serum immunosuppressive factors of tumor bearer, the, effects of normal C3H/ He mice serum (NMS), serum from methylcholanthrene induced fibrosarcoma bearing mice (F4w serum) and sera fractionated by high performance liquid chromatography (HPLC) using anion exchange column were examined on mitogen-induced blastogenesis of normal murine spleen cells (Nspc).
The addition of 5％ of NMS suppressed the blastogenesis of Nspc. Less than 0.6％ of F4w serum suppressed the blastogenesis. NMS was applied to HPLC and was separated to four peaks (A, B, C, D). In HPLC pattern of F4w serum, peak B was higher than that of NMS and new peak (E) was observed next to peak D. Peak D of NMS suppressed the blastogenesis. Not peak D but peak E of F4w serum suppressed the blastogenesis. The properties of those blastogenesis-suppressive fractions were discussed.

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