J.Jpn. Surg. Soc.. 86(7): 787-801, 1985

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Original article

A BASIC STUDY ON ANTI-TUMOR ACTIVITY OF PERIPHERAL LYM-PHOCYTES OF TUMOR-BEARING RATS BY IN VITRO CULTURE

In order to establish passive cancer immunotherapy by in vitro culture of lymphocytes, several methods were devised to induce and augment anti-tumor activity of peripheral blood lymphocytes (PBL) in the presence of interleukin-2 (IL-2).
[I] In vitro experiment
(1) Weak anti-tumor activity appeared on culture of PBL in the presence of IL-2.
(2) On addition of MMC treated tumor antigen, the anti-tumor activity was augmented.
(3) Anti-tumor activity further increased on addition of peritoneal exudate macrophages or peripheral whole white blood cells containing monocytes to (2) as accessory cells.
(4) Effect similar to (3) was obtained in MLTC of peripheral whole white blood cells.
[II] In Winn neutralization test, the strongest tumor-neutralizing activity was seen in the group of cultured lymphocytes with antigen and in the group of cultured leukocytes with antigen. This result agreed excellently with the results of the in vitro experiments [I]．
[III] In the therapeutic experiment
(1) Significantly better effect was noted in the treated group by cultured leukocytes with antigen than in the treated group by non-cultured lymphocytes.
(2) The treatment with 10⁷ lymphocytes produced definitely prolonged survival of tomor-bearing rats compared to the treatment of 10⁶ or less number lymphocytes.

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