J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 7101KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 86(1): 8-22, 1985

Original article

A IN VITRO SENSTITIVITY ASSAY OF ANTI-CANCER AGENTS BY MEASUR-ING THE INHIBITION RATE OF DNA SYNTHESIS (3H-THYMIDINE UPTAKE) OF CANCER CELLS－CLINICAL STUDY

The Second Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan

Yoshinori Nio, Takashi Inamoto, Kazuhisa Ohgaki, Norimichi Kan, Taisuke Hori, Noboru Nakayama, Nobuyasu Yamasaki, Yorinori Hikasa

In this paper, we demonstrated a selection system of anti-cancer agents (ACA) using discontinuous Ficoll density gradient method (DFDGM) for purification of tumor cells and 3H-Thymidine for evaluation of DNA synthesis of tumor cells.
The tumor cells, purified to more than 80％ by DFDGM, were contacted with ACAs for 3 days from the culture initiation and tumor suppression rate (TSR) by ACAs were calculated by following formula ;
TSR=(ACA(－)cpm－ACA(＋)cpm－background cpm)/(ACA(－)cpm－background cpm)×100% 7 ACAs; Mitomycin C (MMC)
Adriamycin (ADM), 5-Fluorouracil (5-FU), Cytosine Arabinoside (Ara-C), Carbazilquinone (CQ), ACNU and Cis-platinum (CDDP) were examined in 106 cancers ; 60 breast cancers, 18 gastric cancers, 23 colorectal cancers and 5 others.
ADM and CQ showed high TSR against breast cancers, CQ against gastric cancers, and 5-FU and CQ against colo-rectal cancers, respectively.
The reliability of this ACA selection system should be evaluated by future clinical studies, however, we stress that ACA should be selected by not only the effect on tumor cells but also the effect on immunity of the host, in future.

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