J.Jpn. Surg. Soc.. 85(8): 799-810, 1984

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Original article

FLOW CYTOMETRIC ANALYSIS OF HEPATOCELLULAR REGENERATION AFTER PARTIAL HEPATECTOMY AND ADMINISTRATION OF HEPATOTROPHIC FACTORS

Kinetics of hepatocellular regeneration after 30% and 70% hepatectomy and the effect of hepatotrophic factors on the normal liver and regenerating livers after hepatectomy were investigated by flowcytometric analysis using adult male rats.
The results were as follows :
1) The DNA histogram of hepatocytes in the normal group had two peaks in G₀G₁ and G₂M components ; G₀G₁ 29% S 13%, G₂M 58%.
2) In the 30% hepatectomized group, the peak of G₂M slightly increased, and remarkable change was not noticed during 4 weeks.
3) In the 70% hepatectomized group, the percentage of hepatocytes in G₀G₁ and G₂M were markedly changed during 2 days after hepatectomy. The peak of G₀G₁ moved to S after 24 hours, to G₂M after 36 hours and returned to G₀G₁ after 48 hours. Afterwards the G₀G₁ gradually decreased while the G₂M increased and they finally reached a single peak of G₂M at the 7th day.
4) Supernatant of the intestinal homogenate after hepatectomy and EGF initiated the cell kinetics in normal and regenerating rat livers.
5) Changes on cell kinetics was not observed after insulin and glucagon administration.

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