J.Jpn. Surg. Soc.. 85(6): 563-572, 1984

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Original article

ESTABLISHMENT OF CYTOTOXIC LYMPHOCYTES AGAINST AUTOLOGOUS CHOLANGIOCARCINOMA FROM THE PATIENT'S BLOOD LYMPHOCYTES CULTURED IN T CELL GROWTH FACTOR (TCGF)

Experimental studies were performed in order to establish autologous adoptive immunotherapy against human solid tumor. When peripheral blood lymphocytes (PBL) from a patient with cholangiocarcinoma (Ch-1) were cultured in the medium supplemented with T cell growth factor for two weeks they proliferated markedly, and more than 100-fold increase in cell number was achieved. These two week-cultured lymphoid cells (CLC_2w) were revealed to consist mostly of cytotoxic T cells and, in a portion, of NK cells, from the examination of markers such as SRBC-rosette formation, peroxidase, surface lg, IgG Fc receptors, C₃ receptors, OKT3 antigen, OKT8 antigen, and so on. In in vitro ⁵¹Crrelease cytotoxicity assay, fresh PBL from the patient with Ch-1 showed no cytotoxicity against autologous Ch-1 cells which had been maintained by serial transplantations to BALB/c nude mice, whereas CLC_2w exerted obvious cytotoxicity against the autologous Ch-1 cells. CLC_2w, however, did not lyse PHA-stimulated autologous lymphoblasts. CLC_2w also prevented growth of the autologous tumor in nude mice. NK cell activity of CLC_2w appeared to be increased as compared with that of fresh PBL from the patient.

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