J.Jpn. Surg. Soc.. 83(10): 1199-1208, 1982

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Original article

STUDIES ON THE DIFFERENCE IN POLYNUCLEATION OF CULTURED HUMAN MAMMARY TUMOR CELLS AND NORMAL GLANDS TREATED WITH CYTOCHALASIN B

Human mammary tumor cells and normal mammary glands were cultivated for studying biological nature of various mammary tumors, and were investigated the difference in the formation of polynuclear cells by administration of cytochalasin B.
Collagenase for tissue dissociation was used. By the use of insuline, human colostrum milk and cholera toxin in culture medium, epithelial cells of mammary tumors and normal mammary glands grew in culture constantly.
When cytochalasin B was applied to the cultures, polynucleation rates increased in all cancer cases (6.1% in average), but showed very low rates in benign tumors and normal mammary glands (less than 1.1% in average).
From these studies, polynucleation rate treated with cytochalasin B was able to utilize the distinguishment of human malignant and benign mammary tumors as a biological means.
This method was useful to study biological nature of the diseases in border line.

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