J.Jpn. Surg. Soc.. 82(6): 566-574, 1981

---

Original article

STUDIES ON TUMOR-SPECIFIC CELLULAR-IMMUNE RESPONSES WITH SOLUBLE TUMOR ANTIGEN IN MICE
PART I. IMMUNOGENICITY OF HYPOTONIC-SALINE EXTRACTS OF TUMOR CELLS

There are several assay methods to demonstrate the cell-mediated immunity to the tumor of cancer patients. In these assays, usually, whole tumor cells have been used as targets or immunogensf or tumor sensitization. Suitable cell suspension or cultures are rather difficult to be prepared from the solid tumors. In order to prepare experimentally stable antigenic extracts easily, soluble tumor antigen was prepared from mouse ascites hepatoma cells by a hypotonic saline extraction methods.
Immunogenicity of the hypotonic saline extracts was estimated by footpad assay for delayed cutaneous hypersensitivity and by lymphocyte blastogenesis to tumor antigen (MLTR). Transplantability of the hepatoma cells was also examined in mice immunized with the soluble extract and BCG.
In footpad assay the immunized mice showed higher response than the non-immunized mice. In micro-MLTR studies, the optimum concentration of hypotonic saline extracts was 1.0 μg/ml and the maximum lymphocyte blastgenesis was observed on the 4th culture day.
There was a significant relationship between the reaction in footpad assay and the degree of lymphocyte blastgenesis. In the immunized mice, tumor-transplantation resistance and servival rate of the mice with growing tumor were higher than those in the non-immunized mice.
There results indicated that hypotonic-saline extracts of tumor cells possess immunogenicity that could induce transplantation immunity when injected with BCG.

---

---

To read the PDF file you will need Adobe Reader installed on your computer.