J.Jpn. Surg. Soc.. 82(4): 386-395, 1981
Original article
AN ULTRASTRUCTURAL STUDY OF RAT LIVER IN ENDOTOXIN SHOCK WITH SPECIAL REFERENCE TO CYTOCHEMICAL ALTERATION OF LYSOSOME
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First Department of Surgery, Nippon Medical School, Tokyo, Japan (Director: Prof. Akiro Shirota) Yoshinobu Sasamoto |
The activity of serum acid phosphatase (ACPase) was disclosed three folds increase in compaison to the control group three hours after ET administration.
Histochemical activity of β-glucuronidase disclosed intense activity in the lysosomes in Kupffer cells and hepatocytes. This activity was decreased six hours after administration of ET.
The ultrastructural investigation of lysosomes of hepatocytes revealed the four subtypes based on the observation using ACPase activity.
Type I: The type I lysosomes reveal electron dense, uniform structure with small configuration without any distinct discernible internal structure.
Type II: The type II lysosomes are slightly larger than the type I and remnant of mitochondria inside is discernible. The enzymatic activity is demonstrated in the outer membrane of lysosomes.
Type III: The type III lysosomes reveal larger configuration with scattered ACPase activity inside.
Type IV: The type IV lysosomes reveal the eccentric dispersion of ACPase with obliteration of the outer membrane. Note presence of myelin figures inside.
The lysosomes of hepatocyte vary from type I to type IV after administration of ET. The type IV lysosomes revealed leakage into micro bile canaliculi and sinusoidal space through Disse's space which consists most outspoken ultrastructural feature two hours after administration of ET.
It was concluded that lysosomal structures are classified into four subtypes based on the histochemical observation of ACPase.
Biochemical significance of these lysosomes in ET shock was fully described in this communication.
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