J.Jpn. Surg. Soc.. 82(4): 305-315, 1981

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Original article

SUPPRESSIVE EFFECT OF SUPERNATANT OF MIXED LYMPHOCYTETUMOR CELL CULTURES (MLTC) ON THE GROWTH OF HUMAN GASTRIC CARCINOMA TRANSPLANTED IN NUDE MICE

Human gastric carcinomas, KSt-1 and St-15, which had been maintained in nude mice, were used as human tumors, and effect of human lymphocytes on the tumor growth was examined. Growth of St-15 in nude mice was temporarily inhibited by injection of human lymphocytes sensitized in MLTC but not by nonsensitized ones. Injection of supernatant of MLTC to tumor-bearing nude mice suppressed the tumor growth markedly. Spleen cells of nude mice injected with MLTC-supernatant showed significantly increased natural killer (NK) activity, as determined by ⁵¹Cr-release assay on YAC, and increased macrophage activity, as determined by inhibition of ³H-TdR uptake by H-2 syngeneic P-815. These results indicate that human lymphocytes sensitized to tumor may recognize allogeneic HLA on tumor cells and temporarily inhibit the tumor growth, and that MLTC-supernatant activates NK cells as well as macrophages in the tumor-bearing nude mice and these activated cells prevent the tumor growth.

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