J.Jpn. Surg. Soc.. 82(1): 75-85, 1981

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Original article

STUDIES ON PRESERVATION OF THE LIVER SLICE

The objective of this study is to develop a liver tissue material applicable for hepatic assist. As the perfusate, Perfluorotributylamine (FC-43), alone or in combination with Waymouth’s tissue culture media (W.M.), was employed to prepare and incubate sliced canine liver and offered good physiological condition in terms of pH, pO₂, pCO₂ and osmolarity. A static or a circulatory system was used to perfusion the tissue, the latter being a prototype of an extracorporeal metabolic device.
GOT and GPT livelsi n the perfusate were less than 72 mKU/gm. of liver up to 24 hours in consecutive 14 incubations. A urea production rate, under ammonia loading, was the highest when a combination of FC-43 and W.M. was employed, being 4.1 mg/gm. of liver in a static and 3.8 mg/gm. of liver in a circulatory systems after 24 hours of incubation. Various biochemical parameters of the perfusate and mitochondrial function of the slice, when examined, indicated maintenance of integrated function of the liver tissue. Changes in amino acids’ levels in the perfusate were converse to those observed in the sera of acute liver failure. Histological examination, finally, revealed well preserved cellular and lobular architectures even after 24 hours of incubation.
The results indicate that the liver tissue material thus prepared is promising to be incorporated in a metabolic device. The prototype is believed to be applicable for a realistic purpose.

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