J.Jpn. Surg. Soc.. 81(11): 1412-1422, 1980

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Original article

STUDIES ON EXTRA CORPOREAL CIRCULATION USING ARTIFICIAL BLOOD (FLUOSOL-DA) IN CALVES

Extracorporeal circulation (ECC) without donor blood has many advantages, not only in reducing the requirement of donor blood but also in preventing several complications such as serum hepati!is and microembolisms. However, there is a fundamental problem to maintain oxygen delivery in high grade hemodilution especially in the cases of pediatric patients. The purpose of this paper is to examine the possibility of utilizing artificial blood as an oxygen carrier in ECC.
Five holstein male calves weighing 60ー100 kg were anesthetized and cannulated into the right common carotid artery and the right external jugular vein. Approximately 72% venous blood was withdrawn via venous drainage tube into the storage bottle just before ECC which was reinfused intravenously after termination of ECC. ECC was performed using 10% Fluosol-DA with bubble oxygenator for 60 min. The flow rate of the perfusion was approximately 60 ml/kg/min and the oxygen flow was three times the flow of the perfusion rate.
Weaning from ECC was smooth in all calves. Three (C₄, C₅, C₆) of five calves have survived for 1ー4 months in excellent condition without any complications. However, other two calves (C2, C3) died of hemolysis and dyspnea which occurred.at 19 hours after ECC. The causes of hemolysis after ECC were suspected to be the preservation technique of the withdrawn blood which was stored in a nonsiliconized bottle and the remaining large volume of Fluosol-DA in the body after ECC which was ove 12.5 g/kg.
The lowest Hct values were 4ー12% and Fct values were 3ー4% during ECC. PaO₂ was maintained approximately 400 mmHg during ECC. There were no remarkable changes in serum electrolytes and osmorality in these experiments.

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