J.Jpn. Surg. Soc.. 81(7): 632-639, 1980
Original article
EVALUATION OF PLASMA ADENOSINE CYCLIC-3',5'-MONOPHOSPHATE FOLLOWING GLUCAGON STIMULATION AS A PREDICTION OF HEPATIC FUNCTION RESERVE
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First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan Yusuke Ikeda |
Adults 168 Wistar strain rats, male, weighing 250-300 g were used. Acute CCl4 poisoning was induced by a single subcutaneous injection of CCl4. These rats were divided into six experimental groups according to CCl4 doses.
After a 24-hour fasting, plasma cAMP following glucagon stimulation was measured. A histological study was performed on the livers in dead cases and excised liver after 68% hepatectomy as well as serum transaminase (S-GOT), lndocyanine green plasma disappearance rate and oral glucose tolerance test (O-GTT).
Plasma cAMP rose promptly up to the highest level within 10 minutes after glucagon stimulation and came down to the base line level within 40 minutes in each group. However, these peaks reached in 10 minutes were different in each hepatic injured group. According to these responses the six experimental groups were divided into three groups, which were normal, slight injured group and severe injured group.
Dead cases after 68% hepatectomy were found only in IIIrd (severe) grade injured group the mortality rate of which was 17 to 50 percent.
Although ICG plasma disappearance rate, S-GOT and O-GTT found useful in evaluating hepatic injury, they were not proved reliable in predicting mortality rate except for ICG plasma disappearance rate.
It is concluded that the plasma cAMP after glucagon stimulation will be a reliable preoperative test for the prediction of postoperative mortality rate and hepatic function reserve.
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