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J.Jpn. Surg. Soc.. 28(9): 1115-1134, 1927


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EXPERIMENTAL FOUNDATION OF JUDGING METHOD OF THE ACTION UPON CELLS OF LIVING ORGANISMS BY NONSPECIAL-ANTIGEN, AS INDICATED BY PHAGOCYTOSIS IN CIRCULATING BLOOD OF TESTANIMALS.
(The 1st Report of the Biological Test as to the Nonspecial-Antigen,)

From the 2nd Sugigal Clinik (in charge of Prof. Dr. H. Suguro) of the Osaka Medical College., 

Shizumi Kuroda M.D.

Brief description of experiments.
Omnadin, one of the Nonspecial-Antigen, parted in Four Quantities (0.08, 0.1, 0.4 and 0.8 ccm) is injected hypodermically into four groups of testing animals, each composed of three guinea-pigs. At 15 minutes after hypodermic injection of Antigen had been finished, 1.0 ccm of Bacteris-Emulsion (amounting ca. 0.0028 ccm of bacteria) of Staphylococcus pyogenus albus caltivated 24 hours on the neutralagar, is transported into V. jugularis of the guinea-pigs.
At six differenses, intervals of each quater, half, 1, 2, 4 and 6 hours after the transportation of Bacteria-Emulsion in V. jugularis, I made the observation of the codition of phagocytosis and the change of the number of phagocytisrns s. 1. (or leucocytes), in every 1.0 cmm of circulating blood.
Results of the experiments.
1. When the quantity of Nonspecial-Antigen (Omnadin) was increased from 0.08 to 0.1 ccm the states of the phagocytosis and the whole number of phagocytisms in 1.0 cmm of blood are also increased with the quantity of Antigen.
2. But, notwithstanding the quantity was increased from 0.1 ccm of Omnadin, and on the contrary, the leucocytes increased in number according to the addition of the quantity.
3. Hence, the reaction which appears in animal bodies by injection of Nonspecial-Antigen, is divided into two sorts. The one indicates "Nonspecial-Antigenicity" (the action upon cells of living organisms or phagocytosis); and the other is the change in number of all phagocytisms. 1., and that is" the toxity of Nonspecial-Antigen" (the poisonous reaction of Nonspecial-Antigen) ;.
4. That is to say, when the quantity of the Nonspecial-Antigen is increased beyond a certain limit (0.1 ccm in the case of Omnadin), its poisonousness alone accompanies the increase of the quantity, nevertheless "the Nonspecialntigenicity " decrases adversely the increas of the quantity.
5. The change of 25 % or so of the activity of the Nonspecial-Antigen (the action upon cells of living organisms or the phagocytosis) can be detected by the indication of the Antigenicity.
6. In the similar way, the flactuation of 18 % or so of the poisonousness of the Nonspecial-Antigen can be detected by meanse of its poisonous reaction.
Conclusion.
In the light of the foregoing results of our experiments, we believe that the experimental foundation of judging the strength of the phagocytosis, and the amount of the total sum of leucocytes, by following the magnitude of the action upon cells of living organisms and the extent of the poisonousness of Nonspecial-Antigen, is firmly established.
No one can scientifically affirm the fact that the Nonspecial-Antigen would act upon cells of living organisms or their protoplasma until he has first setablished "The judging method" of the action of Nonspecial-Antigen upon cells of living organisms by investigating the relation between its poisonousness and its action.
This conception may henceforth prove to be very influential a factor in the examination of these points, and my results of the experiments are exactly in accord with the results of prof. Dr. H. Suguro's experiments by Special-Antigen. (H. Suguro, Úber die Impedinerscheinung bei Phagozytose. III. Mitteilung. Zeitschr. f. Immunitatsforschung u. exp. Therapie. Bd. 46. S. 399. 1926).
(Authers report.)


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