J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 1022KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 95(3): 192-199, 1994

Original article

ROLE OF NEUTROPHILS IN WARM RENAL ISCHEMIA AND REPERFUSION INJURY IN THE RAT

1) Department of Surgery and Organ Transplantation，The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
2) Numazu City Hospital, Numazu, Japan

Shinji Tomikawa1), Nobuo Akiyama2), Takeshi Nagao1), Hisanori Uchida1)

This study attempts to determine whether the circulating neutrophils play a role in mediating injury resulting from renal ischemia, using rats treated with cyclophosphamide (CY) and/or granulocyte colony･stimulating factor (G-CSF).
The neutrophil counts immediately before 60-minute ischemia decreased by 89% in the CY-treated rats in comparison with the untreated animals (p＜0.01). In the rats given G-CSF with CY, the neutrophil counts significantly increased over that of the CY-treated rats (p＜0.01). The serum creatinine level 24 hours after reperfusion amounted to 4.42±0.37mg/dl in the control rats and 2.63±0.29mg/dl in the CY-treated rats (p＜0.01). In 19 rats,10 controls and the 9 CY-treated, the neutrophil counts immediately before ischemia correlated well with the serum creatinine levels 24 hours after reperfusion (r=0.597; p＜0.01).
Our results appear to indicate that neutrophils play an important role in warm ischemia and reperfusion injury of the kidney.

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