J.Jpn. Surg. Soc.. 83(5): 468-472, 1982

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Original article

HEPATOCELLULAR TRANSPLANTATION

Using rats of inbred strain we studied the ability of dispersed hepatocytes. Hepatocytes was dispersed by Berry & Friend’s method.
Experiment I : Hepatocytes (1×10⁷) suspension prepared from rat liver, was transplanted into the spleen (group A), the pancreas (group B), the peritoneum (group C) and Gastric wall (group D). Histological examination of the spleen was performed one week, three weeks and 6 months after inoculation.
Experiment II : Insulin (15 unit) or glucagon (1 unit) was administered into the hepatocytes suspension. This cell suspension was inoculated into the organ as same as those Experiment I. Animals were classified into eight groups (group E-L).
Experiment III : Animals were classified into four groups. Hepatocytes suspension (1×10⁷/0.3ml) was transplanted into the spleen after resection of caudate lobe (resected ratio 10%) of the liver (group M), caudate+right lateral lobes (resected ratio 30%) (group N), caudate+right lateral+left lateral lobes (resected ratio 60%) (group 0), caudate + right lateral+ left lateral + median lobes (resected ratio 70%). 1) the dispersed transplanted Hepatocytes could live only in the spleen and after 3 weeks formed large cell mass. 2) Some hepatocytes survived and other hepatocytes were phagocyted in the spleen. 3) insulin and glucagon, when they were used individually, was not effective for the growth of the transplanted hepatocytes. 4) according to the resected range of the liver, hepatocytes in the spleen could grow-up actively.

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